Thermo Fisher Scientific CD42b Monoclonal Antibody (HIP1), PerCP-eFluor 710, eBioscience
인간 CD42b 단백질을 인식하는 HIP1 클론 단일클론 항체로, PerCP-eFluor 710 형광체가 결합되어 있습니다. Flow cytometry에 최적화되어 있으며, 488 nm 레이저로 여기되어 710 nm에서 방출됩니다. 혈소판 활성화 및 응고 연구용으로 적합합니다.
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD42b Monoclonal Antibody (HIP1), PerCP-eFluor 710, eBioscience
Thermo Fisher Scientific CD42b Monoclonal Antibody (HIP1), PerCP-eFluor 710, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.5 µg)/test
Publications: View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-eFluor™ 710, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HIP1 |
| Conjugate | PerCP-eFluor™ 710 |
| Excitation / Emission Max | 482 / 708 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2762457 |
Additional Formats
- Unconjugated
- APC
- FITC
- NovaFluor Blue 510
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Yellow 610
- PE
- PE-Cyanine7
- Super Bright 436
- Super Bright 600
- Super Bright 702
- Request custom conjugation
Product Specific Information
Description:
The HIP1 monoclonal antibody reacts with the human CD42b molecule, a 135 kDa transmembrane protein also known as platelet gpIb.
Applications Reported:
HIP1 antibody is reported for use in flow cytometric analysis.
Applications Tested:
Pre-titrated and tested by flow cytometric analysis of human platelets. Recommended usage: 5 µL (0.5 µg) per test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test.
Fluorochrome Information:
PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm). Recommended filter: 710/50 bandpass (695/40 acceptable alternative). Ensure instrument compatibility.
Light Sensitivity:
Sensitive to photo-induced oxidation. Protect vial and stained samples from light.
Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-57) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.
Excitation / Emission / Laser:
488 nm / 710 nm / Blue Laser
Target Information
CD42b is a platelet activation marker involved in coagulation and platelet aggregation. It forms part of the GPIb-IX-V receptor complex, which mediates platelet adhesion to thrombogenic surfaces via interaction with von Willebrand factor (VWF) and other molecules.
CD42b is expressed on platelets and megakaryocytes in bone marrow. Mutations in CD42b are associated with Bernard-Soulier syndrome and platelet-type von Willebrand disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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