Thermo Fisher Scientific F(ab`)2-Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, HRP

Applications and Tested Dilution

Product Specifications

Product Specific Information

Rehydrate with 0.55 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Store lyophilized material at 2–8°C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20°C as a liquid.

Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin rat serum proteins or IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit, or sheep.

Target Information

Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins. They are useful for detection, sorting, or purification of rat antibodies.
Secondary antibodies provide increased versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
They are typically generated by immunizing the host animal with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation) to ensure high specificity.

Available Conjugate Formats

• Alexa Fluor 488
• Alexa Fluor 555
• Alexa Fluor 568
• Alexa Fluor 594
• Alexa Fluor 647
• Alexa Fluor Plus 405
• Alexa Fluor Plus 488
• Alexa Fluor Plus 555
• Alexa Fluor Plus 594
• Alexa Fluor Plus 647
• AP
• Biotin
• FITC
• TRITC
• Custom conjugation available upon request

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.