Thermo Fisher Scientific F(ab`)2-Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, TRITC
TRITC로 결합된 염소 유래 항토끼 IgG(F(ab')2) 2차 항체로, 면역세포염색(ICC/IF)에 적합합니다. 교차흡수 처리로 비특이적 반응을 최소화하였으며, 552/578 nm의 형광 특성을 가집니다. 동결건조 형태로 제공되며, 높은 순도의 친화성 정제 항체입니다.
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Applications
- Immunocytochemistry (ICC/IF): 4 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rabbit |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | TRITC |
| Excitation / Emission Max | 552 / 578 nm |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2536010 |
| Target | IgG |
| Cross Adsorption | Against bovine, human and mouse IgG and serum proteins |
| Antibody Form | F(ab`)2 Fragment |
Product Specific Information
- Rehydrate with 0.55 mL of deionized water and let stand 30 minutes at room temperature to dissolve.
- Centrifuge to remove any particulates.
- Prepare fresh working dilution daily.
- 1 year from date of receipt.
- Based on Immunoelectrophoresis, no reactivity is observed to non-immunoglobulin rabbit serum proteins, or serum proteins from bovine, human, or mouse, IgG from human or mouse.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in detection, sorting, or purification of its specified target.
Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody.
Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Additional Available Conjugates
- Alexa Fluor 350, 405, 430, 488, 514, 532, 546, 555, 568, 594, 633, 635, 647, 660, 680, 700, 750, 790
- Alexa Fluor Plus 488, 555, 594, 647
- AP, APC, Biotin, Biotin-XX
- Cyanine3, Cyanine5
- DyLight 350, 405, 488, 550, 594, 633, 650, 680, 755, 800
- FITC, HRP, Pacific Blue, Pacific Orange
- PE, PE-Alexa Fluor 610, PE-Alexa Fluor 647
- Rhodamine, Rhodamine Red-X, Texas Red, Texas Red-X
- Custom conjugation available upon request
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