Thermo Fisher Scientific Phospho-ZAP-70 (Tyr493) Polyclonal Antibody
ZAP-70 단백질의 Tyr493 인산화 형태를 특이적으로 인식하는 폴리클로날 항체. Western blot, IHC, ICC/IF에 적합. 사람, 생쥐, 랫드 반응성. PBS/glycerol buffer에 보관되며 -20°C에서 안정적. 연구용으로만 사용 가능.
- 판매단위
- pk
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human ZAP70 (Accession P43403), corresponding to amino acid residues around phosphorylated Tyr493. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816375 |
Product Specific Information
This antibody detects endogenous levels of ZAP70 only when phosphorylated at Tyrosine 493.
Target Information
ZAP-70 is a cytosolic protein tyrosine kinase belonging to the Syk family, expressed exclusively in T-cells and natural killer cells. It is essential for T-cell receptor (TCR) activation. Upon TCR engagement, ZAP70 becomes phosphorylated at tyrosines 292, 315, and 319 within the interdomain B region between the SH2 and kinase domains. Phosphorylation at Tyr315 (Vav binding site) and Tyr319 (Lck binding site) enhances ZAP70-mediated lymphocyte signaling, while Tyr292 phosphorylation attenuates activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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