Thermo Fisher Scientific Phospho-C/EBP alpha (Thr226) Polyclonal Antibody
C/EBP alpha의 Thr226 인산화 형태를 특이적으로 인식하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC/IF에 사용 가능. Human, Mouse, Rat 반응성. 1 mg/mL 농도의 액상 제품으로 -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human CEBPA (Accession P49715), corresponding to amino acid residues around phosphorylated Thr226 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2817007 |
Product Specific Information
This antibody detects endogenous levels of C/EBP alpha only when phosphorylated at Thr226.
Target Information
The C/EBP (CCAAT/enhancer binding protein) family of transcription factors—alpha, beta, delta, and epsilon—are widely expressed proteins found in various tissues. These domains contain an evolutionarily conserved five-amino acid motif (the regulatory domain motif RDM) that conforms to the consensus sequence (I/V/L)KXEP.
C/EBP transcription factors play major roles in metabolic regulation in liver and adipose tissues. Studies suggest they act in a cascade aiding in the development of adipose cells. The three C/EBP beta isoforms (LAP*, LAP, and LIP) arise from different translation initiation sites and display distinct functions in gene regulation. C/EBPs are also implicated in nerve growth factor mediation, synaptic plasticity, and inflammatory response in Alzheimer’s disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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