Thermo Fisher Scientific CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH5), Brilliant Ultra Violet 615, eBioscience

Thermo Fisher Scientific CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH5), Brilliant Ultra Violet 615, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.125 µg/test
Publications: -

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 660
• APC
• Biotin
• Brilliant UV 661
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 650
• Brilliant Violet 786
• Functional Grade
• NovaFluor Red 700
• NovaFluor Red 755
• NovaFluor Yellow 690
• NovaFluor Yellow 700
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 780
• Request custom conjugation

Product Specific Information

The MIH5 monoclonal antibody reacts with mouse B7-H1 (PD-L1), a member of the B7 family with a predicted molecular weight of ~40 kDa. It is expressed on most leukocytes (T, B, NK, DC) and acts as a ligand for PD-1, inhibiting T and B cell responses. MIH5 is a blocking antibody.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤0.125 µg/test (100 µL final volume). Cell number may range from 10⁵–10⁸ cells/test. Titrate for optimal performance.

Fluorochrome Information:
Brilliant Ultra Violet™ 615 (BUV615) emits at 615 nm and is intended for use with ultraviolet (355 nm) laser-equipped cytometers. Ensure instrument compatibility.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.

Light Sensitivity:
Protect vial and stained samples from light to prevent photo-induced oxidation.

Fixation:
Samples can be stored in IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.

Excitation: 350 nm
Emission: 615 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

Target Information

Programmed death receptor ligand 1 (PD-L1, B7-H1) is a B7 family member that binds PD-1, negatively regulating TCR signaling. PD-L1 decreases TCR-mediated proliferation and cytokine production, and its expression is abundant on many murine and human cancers, up-regulated by IFN-γ stimulation. PD-L1 may play a key role in tumor immune evasion.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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