Thermo Fisher Scientific alpha-2c Adrenergic Receptor Monoclonal Antibody (N330A/80)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100

-

Product Specifications

Species Reactivity

Human, Mouse

Published species

Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

N330A/80

Immunogen

Synthetic peptide amino acids 442- 462 (QDFRRSFKHILFRRRRRGFRQ, cytoplasmic C-terminus) of human Alpha-2C adrenergic receptor if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:alpha-2c Adrenergic Receptor,uniProtId:P18825-1,ncbiNodeId:9606,antigenRange:442-462,antigenLength:462,antigenImageFileName:MA5-27724_alpha-2c_Adrenergic_Receptor_P18825-1_House_mouse.svg,antigenImageFileNamePDP:MA5-27724_alpha-2c_Adrenergic_Receptor_P18825-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• APC
• FITC
• PE
• PerCP
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2735109

Product Specific Information

1 µg/mL of MA5-27724 was sufficient for detection of Alpha2C Adrenergic Receptor in 20 µg of COS cells transiently transfected with HA-tagged Alpha- lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. |Detects 50kDa or larger (possibly due to dimerization). Does not cross-react with other adrenergic receptors.

This antibody was formerly sold as clone S330A-80.

Target Information

Alpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. The mouse studies revealed that both the alpha2A and alpha2C subtypes were required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons. The alpha2A subtype inhibited transmitter release at high stimulation frequencies, whereas the alpha2C subtype modulated neurotransmission at lower levels of nerve activity. This gene encodes the alpha2C subtype, which contains no introns in either its coding or untranslated sequences.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.