Thermo Fisher Scientific Ankyrin 1 Monoclonal Antibody (N388A/10)
Ankyrin 1 단백질을 검출하기 위한 Mouse monoclonal antibody (Clone N388A/10). Western blot, IHC, ICC/IF에 적합하며 Human, Mouse, Rat 시료에 반응. Protein G 정제, PBS/glycerol buffer, -20°C 보관.
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Thermo Fisher Scientific Ankyrin 1 Monoclonal Antibody (N388A/10)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:100 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N388A/10 |
| Immunogen | Fusion protein amino acids 1–1881 (full-length) of human Ankyrin-R |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2735111 |
Additional Formats Available
Product Specific Information
A 1:100 dilution of MA5-27723 was sufficient for detection of Ankyrin R in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 200 kDa. Does not cross-react with Ankyrin-B or Ankyrin-G.
This antibody was formerly sold as clone S388A-10.
Target Information
Ankyrins are a family of proteins that link integral membrane proteins to the spectrin-actin cytoskeleton, playing key roles in cell motility, activation, proliferation, and maintenance of specialized membrane domains. Multiple isoforms are expressed in a tissue-specific, developmentally regulated manner. Ankyrin 1, the prototype, was first discovered in erythrocytes and is also found in brain and muscles. Mutations in erythrocytic ankyrin 1 are associated with hereditary spherocytosis. Alternative splicing gives rise to various isoforms, including truncated muscle-specific forms.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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