Thermo Fisher Scientific NBN Monoclonal Antibody (UMAB99), UltraMAB
Human NBN 단백질을 표적으로 하는 Mouse IgG1 단클론 항체로, Western blot, IHC, ICC, ChIP 등 다양한 분석에 사용 가능. 동결건조 형태로 제공되며, PBS 완충액과 trehalose 포함. 연구용으로만 사용.
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Applications
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | Assay-dependent | |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | |
| Immunocytochemistry (ICC/IF) | 1:100 | |
| ChIP assay (ChIP) | Assay-dependent | |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | UMAB99 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 461–754 of human NBN produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The p95 gene (identical to NBS1 and nibrin) is a member of the hMre11/hRad50 double-strand break complex. This complex is implicated in Nijmegen breakage syndrome, an autosomal recessive disorder characterized by increased cancer incidence, cell cycle checkpoint defects, and sensitivity to ionizing radiation. This reveals a direct molecular link between double-strand break repair and cell cycle checkpoint functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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