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Thermo Fisher Scientific OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal Antibody (eBio25-D1.16 (25-D1.16)), Biotin, eBioscience
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Thermo Fisher Scientific

Thermo Fisher Scientific OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal Antibody (eBio25-D1.16 (25-D1.16)), Biotin, eBioscience

상품 한눈에 보기

OVA257-264(SIINFEKL) 펩타이드가 H-2Kb에 결합된 복합체를 인식하는 단클론 항체로, 항원제시세포(APC) 추적에 유용. Flow cytometry 및 IHC에 적합하며, Biotin으로 표지됨. 마우스 IgG1, kappa 아이소타입. 연구용으로만 사용 가능.

카탈로그번호
13-5743-8x (2개 옵션)
브랜드
Thermo Fisher Scientific
카테고리
Monoclonal Antibodies
판매단위
pk

카탈로그

2개 옵션
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마지막 업데이트 2025. 08. 05. 오후 03:03
Thermo Fisher Scientific 13-5743-82 OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal Antibody (eBio25-D1.16 (25-D1.16)), Biotin, pk판매 단위 pk ·
재고 확인 필요
489,800원VAT 포함 538,780원
Thermo Fisher Scientific 13-5743-81 OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal Antibody (eBio25-D1.16 (25-D1.16)), Biotin, pk판매 단위 pk ·
재고 확인 필요
231,700원VAT 포함 254,870원
재고문의·견적요청 옵션은 실시간 상담으로 납기를 확인해 드립니다 · 연구비 카드 결제 가능

Thermo Fisher Scientific · Thermo Fisher Scientific OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal Antibody (eBio25-D1.16 (25-D1.16)), Biotin, eBioscience

Applications and Tested Dilution

Application Tested Dilution Publications
Immunohistochemistry (IHC) Assay-Dependent -
Flow Cytometry (Flow) 0.125 µg/test 12 publications
Control (Ctrl) - 1 publication

Product Specifications

항목 내용
Species Reactivity Mouse
Published Species Mouse
Host / Isotype Mouse / IgG1, kappa
Recommended Isotype Control Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Biotin, eBioscience™
Class Monoclonal
Type Antibody
Clone eBio25-D1.16 (25-D1.16)
Conjugate Biotin
Form Liquid
Concentration 0.5 mg/mL
Purification Affinity chromatography
Storage Buffer PBS, pH 7.2
Contains 0.09% sodium azide
Storage Conditions 4°C, store in dark, DO NOT FREEZE
Shipping Conditions Ambient (domestic); Wet ice (international)
RRID AB_1210601
Filtration 0.2 µm post-manufacturing filtered

Available Formats

Product Specific Information

Description:
The 25-D1.16 monoclonal antibody reacts with the ovalbumin-derived peptide SIINFEKL bound to H-2Kb of MHC class I, but not with unbound H-2Kb or H-2Kb bound with irrelevant peptides. This antibody is useful for tracking the quantity and localization of specific antigen-presenting cells (APC) in vivo.

Applications Reported:
This eBio25-D1.16 (25-D1.16) antibody has been reported for use in flow cytometric analysis and immunohistochemical staining.

Applications Tested:
Tested by flow cytometric analysis of SIINFEKL-pulsed C57Bl/6 splenocytes. Recommended ≤0.125 µg per test (100 µL final volume). Cell number may vary from 10⁵ to 10⁸ cells/test. Optimize titration for best performance.

Cell Pulsing Protocol with SIINFEKL Peptide:

  1. Add SIINFEKL peptide to cells in flow staining buffer to a final concentration of 30 µM.
  2. Incubate at 37°C for 2 hours.
  3. Wash cells with flow staining buffer.
  4. Proceed with normal cell surface staining.

For additional information, refer to the listed references.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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