
Thermo Fisher Scientific Phospho-SMAD3 (Ser423, Ser425) Monoclonal Antibody (S.434.0)
Phospho-SMAD3 (Ser423, Ser425) 단일클론 항체로 인간, 마우스, 랫트에 반응. Western blot, IP, ChIP 등 다양한 응용 가능. 고순도 친화 크로마토그래피 정제 및 -20°C 보관. 다른 SMAD 패밀리 멤버와 교차 반응 없음.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 3 publications |
| Immunoprecipitation (IP) | 1:50 | - |
| ChIP assay (ChIP) | 1:100 | View 1 publication |
| Gel Shift (GS) | - | View 1 publication |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | S.434.0 |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding pSer423/425 of Smad3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1387 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_11001598 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with other family members.
Target Information
SMAD3 belongs to the SMAD family of proteins, similar to the gene products of the Drosophila gene mothers against decapentaplegic (Mad) and the C. elegans gene Sma.
SMAD proteins act as signal transducers and transcriptional modulators mediating multiple signaling pathways.
SMAD3 functions as a transcriptional modulator activated by transforming growth factor-beta (TGF-β) and may play a role in carcinogenesis regulation.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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