Thermo Fisher Scientific CD44/HCAM Std. Recombinant Mouse Monoclonal Antibody (rHCAM, 918)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

CD44 cell surface antigen is a 100 kDa type I transmembrane glycoprotein widely expressed on human leukocytes, white matter of the brain, and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues.

CD44 acts as a receptor for hyaluronic acid (HA) and is involved in cell–cell interactions, adhesion, and migration. It participates in lymphocyte activation, recirculation, and homing. CD44 expression may be upregulated in certain carcinomas and may be related to metastatic potential.

It is expressed by hematopoietic and non-hematopoietic cells, epithelial tissues, and filopodia in cultured keratinocytes. Bone marrow myeloid cells and memory T cells express CD44 at high levels, while peripheral B and T cells can upregulate CD44 expression upon stimulation. Alternative splicing of the CD44 gene results in multiple isoforms with distinct functions. Dysregulation of CD44 has been associated with diseases such as superficial keratitis and lichen sclerosus, and may play a role in tumor metastasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.