Thermo Fisher Scientific CD25 (soluble) Polyclonal Antibody, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
ELCDDDPPEI PHATFKAMAY KEGTMLNCEC KRGFRRIKSG SLYMLCTGNS SHSSWDNQCQ CTSSATRNTT KQVTPQPEEQ KERKTTEMQS PMQPVDQASL PGHCREPPPW ENEATERIYH FVVGQMVYYQ CVQGYRALHR GPAESVCKMT HGKTRWTQPQ LICTGEMETS QFPGEEKPQA SPEGRPESET SCLVTTTDFQ IQTEMAATME TSIFTTEYQ.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human sIL-2 Receptor alpha. Anti-Human sIL-2 Receptor alpha-specific antibody was purified by affinity chromatography employing an immobilized Human sIL-2 Receptor alpha matrix.

Sandwich ELISA:
To detect Human sIL-2 Receptor alpha by sandwich ELISA (using 100 µL/well), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human sIL-2 Receptor alpha (500-P22RBT) as a detection antibody, allows detection of at least 2000–4000 pg/mL of Recombinant Human sIL-2 Receptor alpha.

Western Blot:
To detect Human sIL-2 Receptor alpha by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human sIL-2 Receptor alpha is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

CD25 (IL-2 receptor alpha chain/IL2RA) is a cytokine receptor involved in the proliferation of T and B lymphocytes. The IL2RA receptor forms a heterotrimeric complex with IL2RB and IL2RG, constituting the high-affinity IL-2 receptor. Homodimeric IL2RA chains yield low-affinity receptors, while IL2RB homodimers yield medium-affinity receptors.
IL2 is primarily produced by mature T cells and functions as a growth factor, differentiation factor, and regulator of apoptosis. It stimulates B cell proliferation, enhances NK cell activity, and inhibits granulocyte macrophage colony formation. Mutations in IL2RA are associated with interleukin 2 receptor alpha deficiency and immune dysregulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.