Thermo Fisher Scientific CD25 (soluble) Polyclonal Antibody, PeproTech
인간 CD25 (IL-2 Receptor α) 인식 토끼 폴리클로날 항체로, Western blot 및 ELISA에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 동결건조 형태로 제공됩니다. 인간 sIL-2Rα 검출에 높은 민감도를 제공합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.5–2.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CHO cells-derived Recombinant Human IL-2 Receptor alpha |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS |
| Contains | No preservative |
| Storage conditions | -20°C |
| Shipping conditions | Ambient |
| RRID | AB_2929933 |
Product Specific Information
AA Sequence of recombinant protein:
ELCDDDPPEI PHATFKAMAY KEGTMLNCEC KRGFRRIKSG SLYMLCTGNS SHSSWDNQCQ CTSSATRNTT KQVTPQPEEQ KERKTTEMQS PMQPVDQASL PGHCREPPPW ENEATERIYH FVVGQMVYYQ CVQGYRALHR GPAESVCKMT HGKTRWTQPQ LICTGEMETS QFPGEEKPQA SPEGRPESET SCLVTTTDFQ IQTEMAATME TSIFTTEYQ.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human sIL-2 Receptor alpha. Anti-Human sIL-2 Receptor alpha-specific antibody was purified by affinity chromatography employing an immobilized Human sIL-2 Receptor alpha matrix.
Sandwich ELISA:
To detect Human sIL-2 Receptor alpha by sandwich ELISA (using 100 µL/well), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human sIL-2 Receptor alpha (500-P22RBT) as a detection antibody, allows detection of at least 2000–4000 pg/mL of Recombinant Human sIL-2 Receptor alpha.
Western Blot:
To detect Human sIL-2 Receptor alpha by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human sIL-2 Receptor alpha is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
CD25 (IL-2 receptor alpha chain/IL2RA) is a cytokine receptor involved in the proliferation of T and B lymphocytes. The IL2RA receptor forms a heterotrimeric complex with IL2RB and IL2RG, constituting the high-affinity IL-2 receptor. Homodimeric IL2RA chains yield low-affinity receptors, while IL2RB homodimers yield medium-affinity receptors.
IL2 is primarily produced by mature T cells and functions as a growth factor, differentiation factor, and regulator of apoptosis. It stimulates B cell proliferation, enhances NK cell activity, and inhibits granulocyte macrophage colony formation. Mutations in IL2RA are associated with interleukin 2 receptor alpha deficiency and immune dysregulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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