Thermo Fisher Scientific CD25 (soluble) Polyclonal Antibody, Biotin, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.2 µg/mL

ELISA

• Tested Dilution: 0.25–1.0 µg/mL

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
ELCDDDPPEI PHATFKAMAY KEGTMLNCEC KRGFRRIKSG SLYMLCTGNS SHSSWDNQCQ CTSSATRNTT KQVTPQPEEQ KERKTTEMQS PMQPVDQASL PGHCREPPPW ENEATERIYH FVVGQMVYYQ CVQGYRALHR GPAESVCKMT HGKTRWTQPQ LICTGEMETS QFPGEEKPQA SPEGRPESET SCLVTTTDFQ IQTEMAATME TSIFTTEYQ

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human sIL-2 Receptor alpha. Anti-Human sIL-2 Receptor alpha-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human sIL-2 Receptor alpha by sandwich ELISA (using 100 µL/well), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human sIL-2 Receptor alpha (500-P22RBT) as a capture antibody, allows the detection of at least 2000–4000 pg/mL of Recombinant Human sIL-2 Receptor alpha.

Western Blot:
To detect Human sIL-2 Receptor alpha by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible development reagents, the detection limit for Recombinant Human sIL-2 Receptor alpha is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Packaging:
500-P22RBT-1MG will be provided as 2 × 500 µg.

Target Information

CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is part of a heterotrimeric complex with IL2R beta (IL2RB) and IL2R gamma (IL2RG), forming the high-affinity IL2 receptor.
Homodimeric IL2RA chains result in low-affinity receptors, while IL2RB homodimers produce medium-affinity receptors.
IL2 is primarily produced by mature T cells and functions as a growth factor, differentiation factor, and regulator of cell death. It stimulates B cell proliferation, enhances NK cell activity, and inhibits granulocyte macrophage colony formation.
Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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