Thermo Fisher Scientific CD18 Polyclonal Antibody, FITC
FITC 형광 표지를 가진 Thermo Fisher Scientific CD18 폴리클로날 항체로, 유세포 분석에 최적화됨. 마우스 Integrin beta 2 항원을 기반으로 제작되어 높은 특이성과 안정성 제공. 4°C 암조건에서 보관하며 냉동 금지. 연구용으로만 사용 가능.
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Applications
Flow Cytometry (Flow)
- Tested Dilution: 10 µL / 1×10⁶ cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CHO-derived recombinant mouse Integrin beta 2 (Gln24–Asn702) |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_2609482 |
Product Specific Information
In direct ELISAs and Western blots, approximately 30% cross-reactivity with recombinant human Integrin beta 2 and recombinant mouse Integrin beta 6 is observed. Less than 5% cross-reactivity is observed with recombinant mouse Integrin beta 1, beta 4, and beta 7.
Target Information
CD18 (Integrin beta 2 subunit) is a 90 kDa type I transmembrane protein expressed on all leukocytes.
It forms heterodimers with CD11 molecules (CD11a–d) to create leukocyte β2 integrins:
- αLβ2 (CD11a/CD18, LFA-1)
- αMβ2 (CD11b/CD18, Mac-1, CR3)
- αXβ2 (CD11c/CD18)
- αDβ2 (CD11d/CD18)
These integrins are essential for leukocyte adhesion and migration.
Deficiency or reduced expression of CD18 leads to leukocyte adhesion deficiency type I (LAD1) and related immune dysfunctions.
CD18 also mediates leukotoxin-induced cytolysis by Mannheimia haemolytica.
Two transcript variants encoding CD18 have been identified.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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