Thermo Fisher Scientific MAP2K1 Monoclonal Antibody (OTI8H1), TrueMAB
MAP2K1(MEK1) 단백질을 인식하는 Mouse monoclonal antibody로, WB 및 ICC/IF에 적합합니다. HEK293T 세포에서 발현된 full-length human MAP2K1을 면역원으로 제작되었습니다. 동결건조 형태로 제공되며, PBS buffer에 8% trehalose를 포함하고 있습니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:200–1:4000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat, Dog, Human, Mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI8H1 |
| Immunogen | Full-length human recombinant MAP2K1 protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
MAP2K1 (MEK1) is a dual-specificity protein kinase involved in mitogen-activated protein kinase (MAPK) cascades, regulating cell growth and differentiation. It is activated by various growth factors, cytokines, and membrane depolarization. Mek1 activation occurs via phosphorylation at serine residues 218 and 222 by Raf1. It plays roles in stress response, apoptosis, and cytokine-induced proliferation. Mutations in MAP2K1 are associated with cardiofaciocutaneous syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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