Thermo Fisher Scientific MAP2K1 Monoclonal Antibody (OTI8A6), TrueMAB
MAP2K1(MEK1)을 인식하는 Thermo Fisher Scientific의 단클론 항체로, WB, IHC(P), ICC/IF에 적합합니다. 인간, 마우스, 개에 반응하며, 정제된 Lyophilized 형태로 제공됩니다. 연구용으로 세포 성장 및 신호전달 연구에 유용합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:4000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI8A6 |
| Immunogen | Full length human recombinant protein of human MAP2K1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, it is recommended to perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
MAP2K1 (MEK1) kinase is a dual-specificity protein kinase involved in mitogen-activated protein kinase (MAPK) cascades that regulate cell growth and differentiation. It is activated by various growth factors, cytokines, membrane depolarization, and calcium influx. Mek1 activation occurs via phosphorylation at serine 218 and 222 residues by Raf1. It plays roles in stress response, apoptosis, and cytokine-induced proliferation. Mutations in MAP2K1 are associated with cardiofaciocutaneous syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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