Thermo Fisher Scientific CXCL2 Polyclonal Antibody, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.2 µg/mL

ELISA

• Tested Dilution: 0.5–2.0 µg/mL
• Publications: 2

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
APLATELRCQ CLQTLQGIHL KNIQSVKVKS PGPHCAQTEV IATLKNGQKA CLNPASPMVK KIIEKMLKNG KSN

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human GRO-beta (CXCL2). Anti-Human GRO-beta (CXCL2)-specific antibody was purified by affinity chromatography employing an immobilized Human GRO-beta (CXCL2) matrix.

Sandwich ELISA:
To detect Human GRO-beta (CXCL2) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human GRO-beta (CXCL2) (500-P104Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human GRO-beta (CXCL2).

Western Blot:
To detect Human GRO-beta (CXCL2) by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human GRO-beta (CXCL2) is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Target Information

Produced by activated monocytes and neutrophils and expressed at sites of inflammation.
Hematoregulatory chemokine that suppresses hematopoietic progenitor cell proliferation in vitro.
GRO-beta(5–73) shows highly enhanced hematopoietic activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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