Thermo Fisher Scientific CD183 (CXCR3) Monoclonal Antibody (CXCR3-173), PE, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.25 µg/test
• Publications: View 23 publications

Product Specifications

Additional Available Formats

• APC
• Biotin
• Brilliant Violet 421
• Brilliant Violet 480
• FITC
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

The monoclonal antibody CXCR3-173 recognizes mouse CD183 (CXCR3), a seven-transmembrane G-protein-coupled chemokine receptor that binds CXCL9 (Mig), CXCL10 (IP-10), and CXCL11 (ITAC). CD183 plays a critical role in CD4 T cell responses to grafts, and CXCR3 knockout mice show compromised allograft rejection.

Expression is found on NK cells, subsets of T lymphocytes and Tregs, with preferential expression on Th1-polarized cells.

The CXCR3-173 antibody affects chemotaxis in response to ligand; ligand presence eliminates staining. In vivo addition delays cardiac and pancreatic islet allograft rejection.

• Reported Applications: Flow cytometric analysis
• Tested Applications: Flow cytometry of mouse splenocytes
• Recommended Usage: ≤ 0.25 µg/test in 100 µL final volume (cell number 10⁵–10⁸ cells/test)
• Excitation: 488–561 nm
• Emission: 578 nm
• Laser Compatibility: Blue, Green, Yellow-Green lasers
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

This gene encodes a G protein-coupled receptor selective for chemokines IP10, Mig, and I-TAC. These chemokines belong to the CXC subfamily, characterized by a single amino acid separating the first two conserved cysteines. Binding induces leukocyte activation, cytoskeletal rearrangement, and chemotactic migration.

Inhibition by Bordetella pertussis toxin suggests coupling to Gi-class G proteins. Signal transduction likely involves pathways common to other chemokine receptors. Chemokine-induced desensitization occurs via phosphorylation-dependent receptor internalization, with subsequent recycling restoring responsiveness.

CXCR3 is prominently expressed in effector/memory T cells and inflamed tissues, where its ligands are locally produced. It is a key target for small-molecule antagonist development for inflammatory disease therapy. Multiple transcript variants exist, encoding different isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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