Thermo Fisher Scientific AMBRA1 Polyclonal Antibody
AMBRA1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF 등 다양한 응용 가능. 인간, 마우스, 랫트 반응성. 1 mg/mL 농도, PBS 버퍼 및 sodium azide 포함. 냉장 또는 냉동 보관 권장.
- 카탈로그번호
- PA520512
- 판매단위
- pk
카탈로그
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Thermo Fisher Scientific AMBRA1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2 µg/mL |
| Immunohistochemistry (IHC) | 5 µg/mL |
| Immunocytochemistry (ICC/IF) | 20 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | An 18 amino acid peptide from near the amino terminus of human Ambra1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long-term storage store at -20°C. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11152400 |
Product Specific Information
- Suggested positive control: rat brain tissue lysate
- PA5-20512 can be used with blocking peptide PEP-0632.
Target Information
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway, is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components.
Beclin-1, a principal regulator of autophagosome formation, is regulated by Ambra1. Ambra1 associates with Beclin-1 through a region near its center as determined by yeast two-hybrid assay.
Null mutations in this gene in mice resulted in embryonic lethality with severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, unbalanced cell proliferation, and excessive apoptotic death.
Furthermore, down-regulation of Ambra1 in cultured cells through RNA interference decreased the level of rapamycin- and nutrient starvation-induced autophagy. Multiple isoforms of Ambra1 are known to exist.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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