Thermo Fisher Scientific CD162 (PSGL-1) Monoclonal Antibody (4RA10), Functional Grade, eBioscience

Applications

Immunocytochemistry (ICC/IF)

• Tested Dilution: Not specified
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Flow Cytometry (Flow)

• Tested Dilution: 0.25 µg/test
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Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 647
• PE
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

This 4RA10 monoclonal antibody reacts with mouse CD162 (P-selectin glycoprotein ligand-1, PSGL-1).
It is validated for use in flow cytometry and reported to block CD162 binding to CD62P and CD62L.

• Applications Reported: Flow cytometric analysis (fluorochrome-conjugated 4RA10 recommended).
• Applications Tested: Tested by flow cytometric analysis of mouse splenocytes.
  • Recommended usage ≤ 0.25 µg per test (100 µL final volume).
  • Cell number may range from 10⁵ to 10⁸ cells/test.
  • Titrate antibody for optimal performance.

Target Information

CD162 (PSGL-1) is a 120 kDa homodimer protein expressed on leukocytes and platelets.
It binds CD62P (P-selectin), CD62L (L-selectin), and to a lesser degree CD62E (E-selectin).
These interactions are crucial for leukocyte adhesion, rolling, and extravasation.
CD162 is also an immune marker of T cell exhaustion in chronic viral infections and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.