Thermo Fisher Scientific CD3 Monoclonal Antibody (17A2), eFluor 660, eBioscience

Thermo Fisher Scientific CD3 Monoclonal Antibody (17A2), eFluor 660, eBioscience

Applications and Tested Dilutions

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395
• Brilliant UV 496
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 650
• eFluor 450
• eFluor 506
• FITC
• Functional Grade
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 600
• Super Bright 702
• Super Bright 780
• Request custom conjugation

Product Specific Information

The 17A2 monoclonal antibody reacts with the mouse CD3 complex. CD3 subunits gamma, delta and epsilon are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 17A2 to CD3 initiates intracellular biochemical pathways resulting in activation and proliferation.

Applications Reported:

• Flow cytometric analysis
• Immunohistochemical staining of frozen tissue sections

Applications Tested:

• Flow cytometric analysis of mouse splenocytes (≤0.25 µg/test, 100 µL final volume)
• Immunohistochemistry on frozen mouse spleen (≤10 µg/mL)
• Titrate antibody for optimal assay performance

Fluorophore Information:
eFluor® 660 replaces Alexa Fluor® 647, emits at 659 nm, excited by red laser (633 nm).
Excitation: 633–647 nm; Emission: 668 nm; Laser: Red Laser.
Filtered post-manufacturing (0.2 µm).

Target Information

CD3 subunit complex is crucial for transducing antigen-recognition signals into T cells and regulating TCR surface expression. CD3 gamma, delta, epsilon, and zeta subunits are structurally related members of the immunoglobulin superfamily encoded on human chromosome 11. These subunits associate with signal transduction molecules through tyrosine-based motifs. CD3 is expressed on 68–82% of peripheral blood lymphocytes and 65–85% of thymocytes, but not on B or NK cells. Defects in CD3 genes are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures.

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