Thermo Fisher Scientific ATM Recombinant Rabbit Monoclonal Antibody (ARC0129)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

ARC0129

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 2619-2939 of human ATM (Q13315) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ATM,uniProtId:Q13315-1,ncbiNodeId:9606,antigenRange:2619-2939,antigenLength:3056,antigenImageFileName:MA5-35332_ATM_Q13315-1_Rabbit.svg,antigenImageFileNamePDP:MA5-35332_ATM_Q13315-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

3 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2849234

Product Specific Information

Immunogen sequence: SVEALCDAYI ILANLDATQW KTQRKGINIP ADQPITKLKN LEDVVVPTME IKVDHTGEYG NLVTIQSFKA EFRLAGGVNL PKIIDCVGSD GKERRQLVKG RDDLRQDAVM QQVFQMCNTL LQRNTETRKR KLTICTYKVV PLSQRSGVLE WCTGTVPIGE FLVNNEDGAH KRYRPNDFSA FQCQKKMMEV QKKSFEEKYE VFMDVCQNFQ PVFRYFCMEK FLDPAIWFEK RLAYTRSVAT SSIVGYILGL GDRHVQNILI NEQSAELVHI DLGVAFEQGK ILPTPETVPF RLTRDIVDGM GITGVEGVFR RCCEKTMEVM R

Target Information

Ataxia-telangiectasia Mutated (ATM) is a protein that belongs to the PI3/PI4 kinase family. Ataxia-telangiectasia is a rare autosomal recessive disorder characterized by progressive neurologic degeneration, immunologic deficiency, and an increased risk of lymphoid cancer. The ATM gene codes for a protein belonging to the phosphoinositide 3-kinase (PI3K) superfamily. ATM phosphorylates proteins instead of lipid and has many downstream targets that act as cell-cycle regulators including: P53, Mdm2, BRCA1, and SMC1. The ATM protein is responsible for repairing double-stranded DNA breaks that occur because of ionizing radiation and other mutagens. The ATM`s C-terminal region has extensive homology to the catalytic domains of phosphatidylinositol 3-kinases (PI3 kinases). Studies have shown that ATM becomes autophosphorylated and upregulated by exposure to ionizing radiation. AT cells are hypersensitive to ionizing radiation, impaired in mediating the inhibition of DNA synthesis and display delays in p53 induction. Further, DNA damage caused by ionizing irradiation activates ATM-kinase, leading to a cascade of kinase reactions that regulate cell cycle, apoptosis, and DNA damage repair. Studies have linked ATM to apoptosis along with Nbs1 and Chk2 in the E2F1 pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.