Thermo Fisher Scientific SMURF2 Recombinant Rabbit Monoclonal Antibody (4H18L17)
Human SMURF2 단백질(aa 151-242)을 인식하는 recombinant rabbit monoclonal antibody. Western blot 및 ICC/IF에 적합. 높은 특이성과 재현성을 제공하며, lot-to-lot consistency 우수. 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1–2 µg/mL
Immunocytochemistry (ICC/IF)
- Tested Dilution: 2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 4H18L17 |
| Immunogen | Protein corresponding to Human SMURF2 (aa 151–242) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2633079 |
Product Specific Information
This antibody is predicted to react with Monkey, Rat, and Rabbit.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning the specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
SMURF2 is an E3 ubiquitin-protein ligase that transfers ubiquitin from an E2 enzyme to targeted substrates. It interacts with SMAD1, SMAD2, and SMAD7 to trigger their ubiquitination and proteasome-dependent degradation. SMURF2 enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression with SMAD1 leads to a significant decrease in SMAD1 protein levels and a smaller decrease in SMAD2 levels.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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