Thermo Fisher Scientific PAX5 Monoclonal Antibody (1H9), APC, eBioscience
인간 및 생쥐 PAX5 단백질을 인식하는 단클론 항체로, APC 형광체로 결합되어 흐름세포분석에 적합합니다. B세포 발달 및 종양 연구에 활용되며, 0.25 µg/test 농도로 사용 권장됩니다. 4°C 암소 보관, 동결 금지.
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific PAX5 Monoclonal Antibody (1H9), APC, eBioscience
Thermo Fisher Scientific PAX5 Monoclonal Antibody (1H9), APC, eBioscience
Applications
- Flow Cytometry (Intracellular Staining)
Tested Dilution: 0.25 µg/test
Publications: View 4 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1H9 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10734230 |
Available Formats
Product Specific Information
The monoclonal antibody 1H9 recognizes both mouse and human Pax5 (BSAP, B cell specific activator protein), a transcription factor of the paired box (pax) family. Pax5 is the only member expressed in hematopoietic cells and plays a key role in B cell lineage commitment and differentiation. Its expression is maintained through most B cell stages and suppressed during plasma cell differentiation.
Pax5 influences heavy chain recombination and regulates expression of various B and non-B cell proteins. It is associated with several neoplasms including diffuse large B cell lymphoma (DLBCL), non-Hodgkin lymphoma, and B-cell ALL. Translocations with elastin, IGH, ETV6, FOXP1, and EVI3 have been reported.
Recommended Buffers: For optimal intracellular staining and flow cytometry, use Foxp3/Transcription Factor Staining Buffer Set
Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Tested in normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set. Recommended ≤0.25 µg/test in 100 µL final volume (10⁵–10⁸ cells/test). Optimize titration for best results.
Excitation: 633–647 nm
Emission: 660 nm
Laser: Red
Filtration: 0.2 µm post-manufacturing filtered
Target Information
PAX5 is a transcription factor of the PAX family, crucial in early development and B-cell differentiation. It acts as a B-cell lineage-specific activator protein (BSAP), expressed during early but not late stages of B-cell maturation. PAX5 expression is also found in the developing CNS and testis, suggesting roles in neural development and spermatogenesis. Mutations in PAX5 are associated with acute lymphoblastic leukemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.