Thermo Fisher Scientific Rhodopsin Monoclonal Antibody (1D4)
Rhodopsin 단백질 검출용 Mouse monoclonal antibody (Clone 1D4). Western blot, IHC, ICC 등 다양한 응용 가능. Bovine, Human, Mouse, Rat 시료 반응. Protein G 정제 및 PBS/BSA buffer에 보관. 시각 수용체 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:100–1:1,000 | 15 |
| Immunohistochemistry (IHC) | - | 7 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | 1 |
| Immunocytochemistry (ICC/IF) | 1:1,000 | 6 |
| ELISA | - | 1 |
| Immunoprecipitation (IP) | Assay-dependent | 2 |
| Dot blot (DB) | - | 1 |
| Affinity Purification (AP) | - | 1 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Rat |
| Published Species | Bovine, Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1D4 |
| Immunogen | Bovine rhodopsin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_325050 |
Product Specific Information
MA1-722 detects rhodopsin from human, mouse, and bovine retinal samples. Clone 1D4 has also been used successfully in Zebrafish. It does not recognize rhodopsin in zebrafish rods but instead labels long double cone outer segments expressing red opsin (Yin, J. et al., 2012).
MA1-722 has been successfully used in Western blot, IHC (P), immunocytochemistry, and immunoprecipitation procedures. By Western blot, this antibody detects an ~40 kDa protein representing rhodopsin from Sf9 cells expressing the bovine gene. Immunocytochemical staining of rhodopsin in human retinal samples results in staining of both rod and cone outer segments.
The MA1-722 immunogen is bovine rhodopsin. The epitope for this antibody has been localized to the C-terminal nine amino acids of bovine rhodopsin known as the 1D4 epitope.
Target Information
Visual pigments such as rhodopsin and porphyropsin are light-absorbing molecules that mediate vision. Rhodopsin consists of an apoprotein (opsin) covalently linked to 11-cis-retinal. This receptor is coupled to the activation of phospholipase C. Porphyropsin consists of opsin covalently linked to 11-cis 3,4-didehydroretinal.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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