Thermo Fisher Scientific GCAP1 Monoclonal Antibody (G2)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 3 publications 3 publications

Immunoprecipitation (IP)

-

View 2 publications 2 publications

Neutralization (Neu)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Human, Non-human primate

Published species

Bovine, Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

G2

Immunogen

Bacterial expressed full length GCAP-1. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GCAP1,uniProtId:P43080-1,ncbiNodeId:9606,antigenRange:1-201,antigenLength:201,antigenImageFileName:MA1-724_GCAP1_P43080-1_House_mouse.svg,antigenImageFileNamePDP:MA1-724_GCAP1_P43080-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2115533

Product Specific Information

MA1-724 detects GCAP-1 from human, non-human primate and bovine samples. This antibody is specific for GCAP-1 and does not react with other isotypes.

MA1-724 has been successfully used in Western blot procedures. By Western blot, this antibody detects a ~23 kDa protein representing GCAP-1 protein on bovine rod outer segment membranes.

The MA1-724 immunogen is bacterial expressed full length GCAP-1.

Target Information

Guanylate cyclase-activating protein is a l Ca(2+)-binding protein that upregulates synthesis of cGMP in photoreceptors. The known mammalian GCAPs are more than 90% similar, consisting of 201 to 205 amino acids, and containing 3 identically conserved Ca(2+)-binding sites. The GUCA1A gene, also termed GCAP1, is transcribed into a single 1.7-kb mRNA species detectable only in the retina. In a 4-generation British family with typical clinical features of autosomal dominant cone dystrophy a tyr99-to-cys mutation) in the GUCA1A gene has been identified. Another family with a pro50-to-leu mutation in GUCA1A demonstrated phenotypic variability ranging from mild photophobia to rod-cone dystrophy. The mutant protein could activate guanylate cyclase 1 (GUCY2D) and displayed similar calcium sensitivity to wildtype protein. However, there was a marked increase in the susceptibility to protease degradation and a reduction in the thermal stability of the pro50-to-leu mutation, which may depress cellular concentration and thereby contribute to retinal cell mortality.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.