Thermo Fisher Scientific POLR2B Polyclonal Antibody
POLR2B 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, ICC, ChIP 등 다양한 응용에 적합. 고순도 항원 친화 크로마토그래피 정제 제품으로, 인간 및 마우스 반응성 확인. 연구용으로만 사용 가능.
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Thermo Fisher Scientific POLR2B Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
| ChIP assay (ChIP) | Assay-dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to amino acids 1–172 of RNA polymerase IIB (Uniprot ID: P30876) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.32 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11153049 |
Product Specific Information
- Recommended positive controls: H1299, BCL-1
- Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (96%), Xenopus laevis (97%), Chicken (97%), Rhesus Monkey (100%), Bovine (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
POLR2B encodes the second largest subunit of RNA polymerase II, responsible for synthesizing messenger RNA in eukaryotes. This subunit, together with other polymerase subunits, forms a structure maintaining contact between the DNA template and the newly synthesized RNA at the enzyme’s active site.
⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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