Thermo Fisher Scientific Transferrin Monoclonal Antibody (HTF-14)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

This antibody recognizes an epitope located in the N-terminal domain of human serum transferrin, a 77 kDa single polypeptide chain glycoprotein belonging to the iron-binding family of proteins. It is synthesized in the liver and consists of two domains, each with a high-affinity reversible binding site for Fe³⁺.

• Does not cross-react with sheep, canine, equine, or bovine samples.
• Suggested positive control: placental tissue.
• Western Blot: non-reducing conditions.
• Functional Application: Blocking.

Target Information

Transferrins (Tf) are approximately 80 kDa blood plasma glycoproteins synthesized by the liver. They are iron-binding transport proteins capable of binding two Fe³⁺ ions along with an anion, usually bicarbonate. Transferrin is responsible for iron transport from absorption and degradation sites to storage and utilization sites.

Under normal physiological conditions, about one-third of total blood transferrin contains bound iron. Measurement of transferrin levels serves as an indicator of iron-carrying capacity and can reveal abnormalities such as anemia, iron overload, or hemochromatosis. Serum transferrin may also play a role in stimulating cell proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.