Thermo Fisher Scientific CD73 Monoclonal Antibody (eBioTY/11.8 (TY/11.8)), NovaFluor Red 755, eBioscience

Applications

Flow Cytometry (Flow)

• Tested Dilution: 0.2 µg/test
• Publications: -

Product Specifications

Additional Formats

• Unconjugated
• eFluor 450
• Functional Grade
• NovaFluor Blue 760
• NovaFluor Red 685
• NovaFluor Red 725
• NovaFluor Yellow 610
• NovaFluor Yellow 690
• NovaFluor Yellow 730
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

Description:
eBioTY/11.8 recognizes CD73, a 69-kDa GPI-anchored cell-surface protein with ecto-5’-nucleotidase activity. Expression on myeloid cells (CD11b) is restricted to the bone marrow. In humans, CD73 can be induced to secrete a soluble form with IL-2, suggesting a role in activation signaling.
CD73 expression increases during lymphocyte maturation and is found on regulatory T cells as well as memory CD4 T cells (Thpp).

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported:

• Flow cytometric analysis

Applications Tested:

• Tested by flow cytometric analysis of mouse splenocytes
• Recommended ≤ 0.2 µg/test (in 100 µL final volume, 10⁵–10⁸ cells/test)
• Titrate for optimal performance

NovaFluor Dyes Compatibility:

• Not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI)
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for best results

CellBlox Plus Blocking Buffer (Cat. No. C001T06F01):

• Required for optimal staining with NovaFluor conjugates
• Add 5 µL per sample before combining with cells
• For single-color controls: 5 µL per 100 µL cell sample (10³–10⁸ cells)

NovaFluor Conjugate Technology:
Based on Phiton™ technology utilizing nucleic acid dye structures engineered for minimized spillover and spread.

• Excitation: 636 nm
• Emission: 756 nm
• Laser: 633–640 nm (Red)

Target Information

CD73 (Ecto-5’-nucleotidase):
Catalyzes conversion of purine 5’-mononucleotides to nucleosides, preferring AMP as substrate.
CD73 is a dimer of two 70 kDa subunits anchored to the plasma membrane via GPI linkage and serves as a lymphocyte differentiation marker.
Deficiency is associated with immunodeficiency diseases and calcification disorders.
Two transcript variants encoding different isoforms have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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