Thermo Fisher Scientific CD73 Monoclonal Antibody (eBioTY/11.8 (TY/11.8)), NovaFluor Red 725, eBioscience

Thermo Fisher Scientific CD73 Monoclonal Antibody (eBioTY/11.8 (TY/11.8)), NovaFluor Red 725, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry
• Tested Dilution: ≤ 0.2 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• eFluor 450
• Functional Grade
• NovaFluor Blue 760
• NovaFluor Red 685
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 690
• NovaFluor Yellow 730
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436 / 600 / 702
• Custom conjugation available

Product Specific Information

eBioTY/11.8 recognizes CD73, a 69 kDa GPI-anchored cell-surface protein with ecto-5'-nucleotidase activity.
CD73 is expressed on subsets of lymphocytes and increases during lymphocyte maturation. It is found on memory CD4 T cells and regulatory T cells, suggesting roles in immune regulation and activation signaling.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis.
Applications Tested: Mouse splenocytes, ≤ 0.2 µg/test (100 µL final volume, 10⁵–10⁸ cells/test).
Titrate antibody for optimal performance.

Important Notes:

• NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.
• Use CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for optimal staining. Add 5 µL per sample to antibody cocktails/master mixes before combining with cells.

Technology:
NovaFluor conjugates utilize Phiton™ technology with engineered nucleic acid dye structures, minimizing spillover and spread.

• Excitation: 636 nm
• Emission: 727 nm
• Laser: 633–640 nm (Red)

Target Information

CD73 (Ecto-5'-nucleotidase) catalyzes conversion of purine 5'-mononucleotides to nucleosides, primarily AMP to adenosine.
It is a dimer of two 70 kDa subunits attached to the plasma membrane via GPI linkage and serves as a lymphocyte differentiation marker.
Deficiency in CD73 is associated with immunodeficiency, joint and artery calcification, and intestinal tuberculosis.
Gene location: 6q14–q21. Two transcript variants encoding different isoforms have been identified.

For Research Use Only. Not for use in diagnostic procedures.

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