Thermo Fisher Scientific ASH2 Polyclonal Antibody
Thermo Fisher Scientific의 ASH2 Polyclonal Antibody는 인간 및 마우스 시료에 반응하며, Western blot, IHC, IP 등 다양한 응용에 적합합니다. 항원 친화 크로마토그래피로 정제된 고순도 항체로, 1 mg/mL 농도의 액상 형태이며 연구용으로 사용됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 225 and 275 of human Absent, Small, or Homeotic-Like 2 (ASH2L) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Phosphate/tris citrate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Target Information
The human ASH2L gene encodes a 628 amino acid protein known as ASH2L1 (isoform 1), which contains a nuclear localization signal and PHD finger motif, suggesting transcriptional regulatory function.
Predicted molecular weight: 68.7 kDa
Alternative splicing may yield isoforms of 60 kDa and 56.5 kDa.
Human ASH2L shares 60% homology with Drosophila ash2, which regulates gene expression during early development, and 90% homology with mouse ASH2L.
Expression is predominant in adult heart and testis, and fetal lung and liver, with minimal levels in adult lung, liver, kidney, prostate, and peripheral leukocytes.
In leukemia cell lines (K562, Hel, Dami), differentiation inducers such as phorbol ester and hemin alter expression patterns, indicating a role in hematopoiesis and leukemia association.
ASH2L also interacts with beta-tubulin and beta-catenin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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