
Thermo Fisher Scientific ASH2 Polyclonal Antibody
Rabbit polyclonal antibody recognizing human ASH2L protein. Validated for WB, IHC, and IP applications. Supplied as a liquid, 1 mg/mL concentration. Recommended storage at 4°C and shipping on wet ice. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 225 and 275 of human Absent, Small, or Homeotic-Like 2 (ASH2L) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Target Information
The human ASH2L gene encodes a 628 amino acid protein (ASH2L1, isoform 1) that contains a nuclear localization signal and a PHD finger motif, suggesting a role as a transcription regulator.
Predicted molecular weight: 68.7 kDa.
Alternative splicing may produce isoforms of 60 kDa and 56.5 kDa.
- Homology: 60% with Drosophila ash2, 90% with mouse ASH2L
- Expression: Predominantly in adult heart and testis, fetal lung and liver; low in adult lung, liver, kidney, prostate, and peripheral leukocytes.
- Function: Involved in gene regulation during development and hematopoiesis; associated with specific leukemia cell lines (e.g., K562, Hel, Dami).
- Interactions: Suggested to interact with beta-tubulin and beta-catenin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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