Thermo Fisher Scientific Phospho-Cyclin B1 (Ser116) Polyclonal Antibody
Cyclin B1의 Ser116 인산화 형태를 특이적으로 검출하는 rabbit polyclonal antibody. Western blot에 적합하며 인간 시료 반응성. 고순도 정제, 1 mg/mL 농도, -20°C 보관. 세포주기 G2/M 조절 연구에 활용 가능.
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Applications
- Western Blot (WB): 1:500–1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human CCNB1 (Accession P14635), corresponding to amino acid residues around phosphorylated Ser116 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816624 |
Product Specific Information
This antibody detects endogenous levels of Cyclin B1 only when phosphorylated at Ser116.
Target Information
Cyclins bind to and regulate the activity of Cyclin-dependent protein kinases (CDKs).
Cyclin B1 (CCNB1) is a regulatory protein involved in mitosis and is essential for controlling the cell cycle at the G2/M transition.
Cyclin B1 complexes with p34 (cdc2) to form the maturation-promoting factor (MPF).
Two alternative transcripts exist: one constitutively expressed and another predominantly expressed during G2/M phase.
Cyclin B1 accumulates steadily during G2 phase and is abruptly degraded at the end of mitosis via APC-dependent destruction.
Its destruction is required for cell cycle progression.
Cyclin B1 is overexpressed in various cancers, including breast, prostate, and non-small cell lung cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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