
Thermo Fisher Scientific ZBED1 Monoclonal Antibody (OTI2E5), TrueMAB
인간 ZBED1 단백질을 인식하는 마우스 단클론 항체로 Western Blot, IHC, Flow Cytometry에 적합. 동결건조 형태로 제공되며 PBS 버퍼에 trehalose 포함. 세포 증식 관련 연구 및 DNA 복제 인자 분석에 활용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2E5 |
| Immunogen | Full length human recombinant protein of human ZBED1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
ZBED1 (also known as DREF) is the human homolog of the Drosophila DREF protein, a transcriptional regulatory factor essential for genes involved in DNA replication and cell proliferation. RNAi experiments targeting ZBED1 RNA inhibited S-phase entry and reduced histone H1 mRNA in HeLa cells, indicating its role in cell proliferation.
ZBED1 binds promoter regions of at least 22 ribosomal protein genes; RNAi against ZBED1 decreases RP gene expression. Immunoprecipitation analyses show ZBED1 self-associates via a carboxyl-terminal hATC domain, required for nuclear localization and DNA binding.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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