Thermo Fisher Scientific IFN gamma Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
MQGTLIESLE SLKNYFNSSS MDAMEGKSLL LDIWRNWQKD GNTKILESQI ISFYLRLFEV LKDNQAISNN ISVIESHLIT NFFSNSKAKK DAFMSIAKFE VNNPQIQHKA VNELIRVIHQ LSPESSLRKR KRSRC

Preparation:
Produced from sera of goats immunized with highly pure Recombinant Rat IFN-gamma. The anti-Rat IFN-gamma-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Rat IFN-gamma by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required.
This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Rat IFN-gamma (500-P122G) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Rat IFN-gamma.

Western Blot:
To detect Rat IFN-gamma by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL.
When used with compatible secondary reagents, the detection limit for Recombinant Rat IFN-gamma is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Packaging:
500-P122GBT-1MG will be provided as 2 × 500 µg.

Target Information

IFN gamma (Interferon gamma, Type II interferon)
IFN gamma is a macrophage activation factor and immune interferon produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, and cytokines.
It is a dimeric glycoprotein of approximately 45 kDa, composed of two 146 amino acid subunits. On SDS-PAGE, it appears as 25, 20, and minor 15.5 kDa bands due to differential glycosylation.

Functions include:

• Antiviral and tumor antiproliferative activity
• Induction of MHC class I and II expression
• Macrophage activation
• Enhanced immunoglobulin secretion by B lymphocytes

IFN gamma activity is species-specific; human IFN gamma does not cross-react with mouse.
It acts through the IFN gamma receptor I and II, activating the JAK-STAT pathway.
Mutations in the IFN gamma gene are associated with aplastic anemia.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.