Thermo Fisher Scientific ST2 Polyclonal Antibody
Thermo Fisher Scientific ST2 Polyclonal Antibody는 인간 및 생쥐 ST2 단백질을 검출하기 위한 고품질 항체입니다. Western blot, IHC, ICC/IF 등 다양한 응용에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific ST2 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | View 2 publications |
| Immunohistochemistry (IHC) | 5 µg/mL | View 5 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 2 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | 20 µg/mL | View publications |
| Flow Cytometry (Flow) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to 16 amino acids at the amino-terminus of mouse ST2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long-term storage, store at -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11156630 |
Product Specific Information
- The immunogenic sequence is common to all three known ST2 isoforms.
- A suggested positive control is 293 cell lysate.
- PA5-20077 can be used with blocking peptide PEP-0195.
Target Information
IL-33R, also known as ST2, is a receptor for IL-33 and a member of the IL-1 receptor family. Its extracellular domain consists of three Ig-like domains that interact with co-receptor IL-1RAcP and ligand IL-33.
IL-33 binding activates signaling pathways including NF-κB and MAPK, leading to cytokine release (IL-5, IL-6, IL-8, IL-13).
Two main isoforms exist: ST2L (membrane-bound) and ST2S (soluble). The soluble form acts as a decoy to dampen IL-33 signaling.
IL-33R is expressed by mast cells, basophils, eosinophils, and in mice by ILC2 and Th2 cells.
IL-33R signaling is implicated in immune conditions such as allergies, arthritis, atherosclerosis, and sepsis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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