Thermo Fisher Scientific RNF216 Polyclonal Antibody
RNF216 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF에 사용 가능하며, 인간 및 마우스 시료에 반응. 1 mg/mL 액상 형태로 제공되며 PBS와 0.02% sodium azide 포함. 냉장 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | View 2 publications |
| Immunohistochemistry (IHC) | 10 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 20 µg/mL | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A peptide corresponding to 15 amino acids near the amino-terminus of mouse TRIAD3A |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long-term storage, store at -20°C. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11155204 |
Product Specific Information
- Suggested positive control: mouse heart tissue lysate
- PA5-20079 can be used with blocking peptide PEP-0197
Target Information
Activation of NF-κB as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses. Signals from these receptors are mediated by adapter molecules such as TRIF, TIRAP, and MyD88.
Regulatory mechanisms control TLR signal transduction, including inhibition by molecules like ST2 and SIGIRR. Another mechanism involves ubiquitinization of selected TLRs by TRIAD3A, an E3 ubiquitin-protein ligase.
TRIAD3A, a RING finger protein, binds to TLR4 and TLR9 (and to a lesser extent TLR3 and TLR5), catalyzing their ubiquitinization. Overexpression of TRIAD3A promotes degradation of TLR4 and TLR9, reducing ligand-specific activation. Conversely, depletion of TRIAD3A enhances TLR activation.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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