Thermo Fisher Scientific CD367 (Clec4A, DCIR) Monoclonal Antibody (9E8), PE, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

9E8

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2572756

Product Specific Information

Description: This 9E8 monoclonal antibody reacts with human CD367, also known as dendritic cell immunoreceptor (DCIR) or Clec4A. This C-type lectin is expressed on monocytes, granulocytes, B cells, and plasmacytoid dendritic cells in peripheral blood. DCIR is also expressed on mature and immature monocyte-derived dendritic cells in vitro. DCIR is not expressed on NK or T cells. With its cytoplasmic ITIM domain, DCIR is an inhibitory receptor that recruits the SHP-1 and SHP-2 phosphatases upon ligand binding. Studies have demonstrated that DCIR plays a role in the interaction between dendritic cells and HIV-1.

Applications Reported: This 9E8 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 9E8 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type 2 transmembrane protein may play a role in inflammatory and immune response. Multiple transcript variants encoding distinct isoforms have been identified for this gene. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.