Thermo Fisher Scientific CellTrace Oregon Green 488 Carboxylic Acid Diacetate, Succinimidyl Ester (Carboxy-DFFDA, SE), cell permeant, mixed isomers

Invitrogen™ CellTrace™ Oregon Green™ 488 Carboxylic Acid Diacetate, Succinimidyl Ester (Carboxy-DFFDA, SE)

Like CFDA SE, CellTrace™ Oregon Green® 488 (carboxy-DFFDA SE) is a useful tool for tracking proliferating cells. This Oregon Green® 488 probe passively diffuses into cells, where it is initially colorless and nonfluorescent. Once inside, intracellular esterases remove the acetate groups, producing a highly fluorescent, amine-reactive dye. Upon reaction with intracellular amines, the probe forms Oregon Green® 488 conjugates that are well retained by cells.

Unlike fluorescein conjugates, Oregon Green® 488 conjugates exhibit bright, photostable green fluorescence that remains stable across physiological pH values (pH 6–8).

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