Thermo Fisher Scientific Plexin-A1 Polyclonal Antibody
Plexin-A1 단백질을 검출하는 Thermo Fisher Scientific의 폴리클로날 항체. WB, IHC, ICC, IP 등 다양한 응용 가능. 인간, 마우스, 랫트 반응성. 항원 친화 크로마토그래피로 정제된 고품질 항체로 신경 성장 및 세포 이동 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:100 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:500 |
| Immunoprecipitation (IP) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Plexin-A1 synthetic peptide (coupled to carrier protein) corresponding to a region within the N-terminal half of the semaphorin domain in human Plexin-A1. Highly conserved in rat and mouse Plexin-A1, low homology to other Plexin family members. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.75 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2942973 |
Product Specific Information
This antibody detects a 220 kDa protein corresponding to the apparent molecular mass of Plexin-A1 on SDS-PAGE immunoblots of neonatal rat brain and Cos-7 cells transfected with mouse Plexin-A1. In immunocytochemistry, it co-localizes with anti-Myc in Cos-7 cells transfected with Myc-tagged mouse Plexin-A1.
Target Information
Coreceptor for SEMA3A, SEMA3C, SEMA3F, and SEMA6D. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth, and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for activation of downstream signaling events in the cytoplasm. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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