
Thermo Fisher Scientific CLCN3 Monoclonal Antibody (N258/5), PE
CLCN3 단백질을 인식하는 단클론 항체로, PE 형광표지되어 세포 분석에 적합합니다. Western blot, IHC, ICC/IF에 사용 가능하며 사람, 생쥐, 랫트 시료에 반응합니다. Protein G로 정제된 액상 항체로 4°C 보관합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N258/5 |
| Immunogen | Synthetic peptide amino acids 98–115 (cytoplasmic N-terminus) of rat Clcn3 |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932011 |
Additional Formats
- Unconjugated (MA5-27709)
- APC (MA5-45554)
- FITC (MA5-45555)
- PerCP (MA5-45556)
- Custom conjugation available upon request
Product Specific Information
- 1 µg/mL of MA5-45557 detects Clcn3 in 20 µg of rat brain membrane lysate (colorimetric immunoblot using goat anti-mouse IgG:HRP).
- Detects approximately 90 kDa.
- Does not cross-react with Clcn4 or Clcn5 (based on KO validation).
- Formerly sold as clone S258-5.
Target Information
CLCN3 mediates chloride/proton exchange, functioning as an antiporter contributing to endosome and synaptic vesicle acidification. It may influence vesicle trafficking, exocytosis, and neuronal excitability regulation by protein kinase C, playing a role in short-term memory formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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