Thermo Fisher Scientific CLCN3 Monoclonal Antibody (N258/5), APC
CLC-3 단백질을 인식하는 마우스 단클론 항체로, APC로 결합되어 세포 분석에 적합합니다. Western blot, IHC, ICC/IF에 사용 가능하며 사람, 마우스, 랫트 반응성이 있습니다. 단백질 G로 정제된 액상 형태로 4°C에서 보관합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N258/5 |
| Immunogen | Synthetic peptide amino acids 98–115 (cytoplasmic N-terminus) of rat Clcn3 |
| Conjugate | APC |
| Excitation/Emission Max | 651/660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932008 |
Additional Formats
- Unconjugated (MA5-27709)
- FITC (MA5-45555)
- PE (MA5-45557)
- PerCP (MA5-45556)
- Request custom conjugation available
Product Specific Information
- 1 µg/mL of MA5-45554 was sufficient for detection of Clcn3 in 20 µg of rat brain membrane lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
- Detects approximately 90 kDa.
- Does not cross-react with Clcn4 or Clcn5 (based on KO validation results).
- This antibody was formerly sold as clone S258-5.
Target Information
CLCN3 mediates the exchange of chloride ions against protons and functions as an antiporter contributing to the acidification of endosomes and synaptic vesicle lumens. It may affect vesicle trafficking and exocytosis and play a key role in neuronal cell function by regulating membrane excitability via protein kinase C. It may also help neuronal cells establish short-term memory.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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