Thermo Fisher Scientific CLCN3 Monoclonal Antibody (N258/5), FITC
CLCN3 단백질을 인식하는 FITC 결합 단클론 항체로, Western blot, IHC, ICC/IF에 사용 가능. 사람, 마우스, 랫트 반응성. 단백질 G 정제, 1 mg/mL 농도. 4°C 암소 보관, 연구용 전용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N258/5 |
| Immunogen | Synthetic peptide amino acids 98–115 (cytoplasmic N-terminus) of rat Clcn3 |
| Conjugate | FITC |
| Excitation/Emission Max | 498/517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage conditions | 4°C, store in dark |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932009 |
Additional Formats
- Unconjugated (MA5-27709)
- APC (MA5-45554)
- PE (MA5-45557)
- PerCP (MA5-45556)
- Custom conjugation available
Product Specific Information
1 µg/mL of MA5-45555 was sufficient for detection of Clcn3 in 20 µg of rat brain membrane lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 90 kDa. Does not cross-react with Clcn4 or Clcn5 (based on KO validation results).
This antibody was formerly sold as clone S258-5.
Target Information
CLCN3 mediates the exchange of chloride ions against protons, functioning as an antiporter contributing to acidification of endosomes and synaptic vesicles. It plays a role in neuronal cell function and may affect membrane excitability and short-term memory formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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