Thermo Fisher Scientific Paxillin Chimeric Recombinant Mouse Monoclonal Antibody (LD2 sAB)
인간 Paxillin LD2 모티프를 인식하는 재조합 마우스 단클론 항체. Western blot과 ELISA에 사용 가능. 높은 특이성과 재현성을 제공하며 Protein A로 정제됨. 단기 4°C, 장기 -20°C 보관 권장.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2 µg/mL |
| ELISA | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | LD2 sAB |
| Immunogen | Peptide corresponding to paxillin-LD2 motif (residues 141–156) fused to MBP; peptide sequence: NLSELDRLLLELNAVQHNP |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3074387 |
Product Specific Information
Specificity: The antibody binds specifically to the LD-2 motif of human paxillin with a Kd of 24 nM.
Target Information
Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as src, FAK, and p210BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. Paxillin associates tightly with FAK and Crk through its SH2 domain, and this interaction is independent of the extracellular matrix. Although paxillin was initially discovered in fibroblasts, its phosphorylation may also be important during neurite extension and differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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