Thermo Fisher Scientific POLR3H Polyclonal Antibody

Thermo Fisher Scientific POLR3H Polyclonal Antibody

Applications and Tested Dilution

• Western Blot (WB): 1:500–1:1,000
• Immunocytochemistry (ICC/IF): 1:100–1:500

Product Specifications

Product Specific Information

Antibody detects endogenous levels of total RPC8.

Target Information

RPC8 is a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into RNA using ribonucleoside triphosphates as substrates. It is a peripheral component of RNA polymerase III, responsible for synthesizing small RNAs such as 5S rRNA and tRNAs.
RPC8 plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. It acts as a nuclear and cytosolic DNA sensor involved in innate immune response, recognizing non-self dsDNA that serves as a template for transcription into dsRNA.
Non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs), induce type I interferon and NF-κB activation through the RIG-I pathway.

Usage Note

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.