Thermo Fisher Scientific POLR3D Polyclonal Antibody
POLR3D 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 Immunocytochemistry에 적합합니다. 인간 및 마우스 시료에 반응하며, Affinity chromatography로 정제된 액상 형태입니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human POLR3D (Accession P05423), corresponding to amino acid residues T281–I331 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2850994 |
Product Specific Information
Antibody detects endogenous levels of total RPC4.
Target Information
This gene encodes a protein that interacts with the N-terminal region of BRCA1. It binds BRCA1 both in vivo and in vitro and shares homology with two conserved BRCA1 regions: the N-terminal RING motif and the C-terminal BRCT domain. The RING motif is a cysteine-rich sequence found in proteins regulating cell growth, including tumor suppressors and proto-oncogenes. This protein also contains three tandem ankyrin repeats. The BARD1/BRCA1 interaction is disrupted by tumorigenic amino acid substitutions in BRCA1, suggesting that their stable complex formation is essential for BRCA1 tumor suppression. This protein may be a target of oncogenic mutations in breast or ovarian cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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