Thermo Fisher Scientific POLR3A Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:1,000

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:100–1:500

Product Specifications

Product Specific Information

Antibody detects endogenous levels of total RPC1.

Target Information

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
POLR3A is the largest and catalytic core component of RNA polymerase III, which synthesizes small RNAs such as 5S rRNA and tRNAs.
It forms the polymerase active center together with the second largest subunit.
A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III.
A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site, promoting translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.