Thermo Fisher Scientific Phospho-RIP3 (Ser232) Polyclonal Antibody
RIP3 단백질의 Ser232 인산화 형태를 특이적으로 인식하는 폴리클로날 항체. Western blot 및 IHC(PFA) 적용 가능. 마우스 및 랫트 반응성. 1 mg/mL 농도의 액상 형태로 제공되며, -20°C에서 보관. 연구용 전용 시약.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 1 publication |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | – | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat |
| Published Species | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from mouse RIP3 around the phosphorylation site of Ser232 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2817129 |
Product Specific Information
Antibody detects endogenous levels of RIP3 only when phosphorylated at Ser232.
Target Information
RIPK3 is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, which contains a C-terminal domain unique from other RIP family members. RIPK3 is predominantly localized to the cytoplasm and specifically colocalized in the mitochondrion, undergoing nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex and can induce apoptosis and weakly activate the NF-kappaB transcription factor. Caspase-8 prevents RIPK3-dependent necrosis without inducing apoptosis by functioning in a proteolytically active complex with CFLAR, which is required for the protective function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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