Thermo Fisher Scientific NSE gamma (Neuron Specific Enolase, gamma) (Neuroendocrine Marker) Monoclonal Antibody (ENO2/4507)
Neuron specific enolase (NSE, gamma) 단클론 항체로 신경 및 신경내분비 세포 마커 검출에 적합. Western blot, IHC(P), IHC(PFA) 등 다양한 응용 가능. Human 시료 반응, Mouse IgG2b 항체. 단백질 A/G 정제, PBS buffer, 보존제 없음.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ENO2/4507 |
| Immunogen | Recombinant fragment of human NSE gamma (around aa416–433) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Neuron specific enolase (NSE, ENO1, ENO2, ENO3) is an enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis and the reverse reaction in gluconeogenesis. NSE is highly stable in biological fluids and diffuses into extracellular medium and cerebrospinal fluid (CSF) when neuronal membranes are injured.
NSE exists as three mammalian isoforms (ENO1, ENO2, ENO3), also known as enolase alpha, beta, and gamma.
- Alpha-subunit: expressed in most tissues
- Beta-subunit: expressed in muscle
- Gamma-subunit: expressed primarily in neurons and neuroendocrine cells
Co-expression of NSE and chromogranin A is common in neuroendocrine neoplasms. Antibodies to NSE are useful for identifying neuronal cell bodies, developing neuronal lineage, and neuroendocrine cells. Release of NSE into CSF and blood is a biomarker of neuronal injury. Clinically, NSE is a sensitive marker for neuronal damage in conditions such as stroke, hypoxic brain injury, status epilepticus, Creutzfeldt-Jakob disease, and herpetic encephalitis. Elevated NSE levels are also seen in neuroblastoma and small cell lung cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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